This study aims to determine the presence of aflatoxin M₁ (AFM₁) in raw cow milk produced in the center and the surrounding villages of Çorum, Turkey. A total of 200 raw milk samples provided directly from the manufactures during four seasons have been analyzed using high performance liquid chromatography (HPLC). AFM₁ was found in 129 out of 200 cow’s milk (64.5%) at levels from 22 to 401 ng/L. Moreover, 87 samples contained AFM1 at levels higher than maximum level of 50 ng/L established by Turkish Food Codex. The contamination of AFM1 level was in the range of 22–289 ng/L in autumn, 29–199 ng/L in winter, 30–177 ng/L in spring, and 26–401 ng/L in summer season. The results show that AFM₁ concentrations carry potential risk from the point of consumers.