Surface contamination of cheese varieties with pathogenic microorganisms is a potential risk in dairy industry. Pulsed UV (PUV) light is a method, which provides rapid inactivation of a wide range of microorganisms on foods. In this study, white cheese inoculated on top surface with Staphylococcus aureus or Escherichia coli O157:H7 was exposed to PUV light. Treatment parameters were time (5, 15, 30, 45, 60 s) and distance from the quartz window of xenon lamp (5, 8, 13 cm). The 60 s-8 cm treatment (91.22 J/cm²) resulted in the highest inactivation level without visually altering the appearance of white cheese. After this treatment, approximately 1.31 and 2.20 log₁₀ reductions (cfu/cm²) were obtained for S. aureus and E. coli O157:H7, respectively. Lipid oxidation, pH, and moisture content, except the color parameters, remained unchanged (p>0.05) after the 60 s-8 cm treatment. Only 1.75 % of the PUV light reached to a depth of 0.5 cm in white cheese after treatment at 5 cm. These findings indicate that although PUV light is able to reduce pathogen counts of white cheese, this technology can only be utilized for surface decontamination.