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1) Animal Production Department, Higher Agronomic Institute of Chott-Meriam, University of Sousse, 4042 Susa Tunisia; 2) Department of Agriculture, Forestry and Food Science, University of Torino, 10095 Grugliasco, Italy

Use of papaya leaf extract for the ­production of fresh goat‘s cheese as an alternative to animal rennet

Verwendung von Papayablattextrakt für die Herstellung von Ziegenfrischkäse als Alternative zu tierischem Lab

Yasser Hachana1), Iheb Frija1), Rabaa Maoual1), Ibrahim El Akram Znaidi1), Riccardo Fortina2)


Carica papaya leaf extract obtained by a simple and chemical-free method was first analyzed for levels of protein, polyphenols, proteolytic activity and milk clotting activity, and then evaluated in fresh goat’s cheese production as an alternative to calf rennet. Milk clotting essays, cheese yields, protein fractions and sensory evaluations were determined considering animal rennet as reference. The highest cheese yield was obtained with 0.2% dose of fresh papaya leaf extract. Compared to calf rennet, papaya leaf extract remained active even at 95 °C. After 12 months of cold storage, the clotting ability of liquid extract decreased significantly, whereas the lyophilized extract maintained its full clotting potential. Cheese samples made with papaya leaf extract showed lower levels of casein but higher levels of pre-α-caseins and water-soluble nitrogen than those made with calf rennet. Sensory results proved that the use of papaya leaf extract in fresh goat’s cheese production had no negative impact on color, consistency or taste, but on the contrary, it improved cheese’s odor which was remarkably appreciated by panelists. This natural product could therefore be a good alternative to conventional animal rennet and could be used to attribute specificity to traditional fresh goat’s cheese.

Keywords: Papaya leaves, calf rennet, goat milk, cheese yield, proteolysis, sensory quality

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Weitere Informationen zum Beitrag

Arch Lebensmittelhyg 72,
185–191 (2021)
DOI 10.2376/0003-925X-72-185


© M. & H. Schaper GmbH & Co.
ISSN 0003-925X

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