1) Scientific Veterinary Institute „Novi Sad“, Rumenački put 20, 21000 Novi Sad, Republic of Serbia; 2) Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21 000 Novi Sad, Republic of Serbia; 3) University of Belgrade, Faculty of Veterinary Medicine, Bulevar oslobodjenja 18, Belgrade, Republic of Serbia
Suzana Vidaković Knežević1), Sunčica Kocić-Tanackov2), Snežana Kravić2), Slobodan Knežević1), Jelena Vranešević1), Radoslava Savić Radovanović3), Nedjeljko Karabasil3)
Fourteen essential oils, including basil, black pepper, cassumunar ginger, cinnamon, lemon, clove, fennel, lavender, myrtle, oregano, rosemary, curry plant, thyme and sage, were screened for their antibacterial activity against important food-borne pathogens, Salmonella Enteritidis and Salmonella Typhimurium. Essential oils have been examined by gas chromatograph coupled to mass spectrometer (GC-MS). The disc diffusion method was used as a screening test for antibacterial activity. Oregano and thyme essential oils showed the greatest inhibition zones against both Salmonella Enteritidis and Salmonella Typhimurium, while black pepper, lemon, curry plant and sage EOs expressed no antibacterial activity against tested Salmonella serotypes. Subsequently, minimal inhibitory concentration and minimal bactericidal concentration were determined by broth microdilution method for all essential oils that showed any inhibition zones (disc diffusion method). The essential oil that showed the highest antibacterial activity against all Salmonella serotypes was oregano, expressing minimal inhibitory concentration values between 0.04 and 0.23 µL/mL, and minimal bactericidal concentration values between 0.09 and 0.45 µL/mL, followed by cinnamon, clove, rosemary and thyme essential oils. The results of this study confirm the antibacterial activity of some essential oils, as well as their potential application as food preservatives.
Keywords: food preservatives, essential oils, foodborne pathogens
Arch Lebensmittelhyg 72,
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